By Didier Montet, R.C. Ray
Aquaculture is at the moment one of many quickest starting to be creation sectors on the planet. It now money owed for almost part (45%) of the world�s foodstuff fish (which refers to construction of aquatic animals: fish, crustaceans, molluscs, amphibians) and this raise is anticipated to arrive 50% in 2015. Genetic engineering and biotechnology have contributed significantly to this box. the main universal equipment in fish biotechnology are chromosome manipulation and hormone remedy which might be used to provide triploid, tetraploid, haploid, gynogenetic and androgenetic fish.
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In vitro activity of the antimicrobial peptide magainin 1 against Bonamia ostreae, the intrahemocytic parasite of the flat oyster, Ostrea edulis. Mol. Mar. Biol. Biotech. 3: 327-333. C. S. (2002). Accelerated growth performance and stable germline transmission in androgenetically derived homozygous transgenic mud loach, Misgurnus mizolepis. Aquaculture 209: 257-270. NAS (National Academy of Science). (2002). Animal biotechnology: science-based concerns. , USA. , Mooney, H. and Troell, M. (2000).
Transgenesis in fish. Experientia 47: 891-897. C. J. (1997). Optimization of the solubilization and renaturation of fish growth hormone produced by Escherichia coli. Appl. Microbiol. Biotechnol. 48: 66-72. , Wang, Y. and Zhu, Z. (2006). A perspective on fish gonad manipulation for biotechnical applications. Chin. Sci. Bull. 51(1): 1-7. , Zou, J. and Zhu, Z. (2007). Antisense of gonadotropin-releasing hormone reduces gonadotropin synthesis and gonadal development in transgenic common carp (Cyprinus carpio).
When expressed, the Cre recombinase excises the transgene in between the 2 loxP sites. The other line of zebrafish contained the T7 RNA polymerase driven by a protamine promoter specifically expressed in the gonad. When these 2 lines of zebrafish are crossed, the offspring specifically express the T7 RNA polymerase in the gonads, resulting in the expression of the Cre recombinase and excision of the foreign gene. This concept would allow for the creation of transgenic individuals lacking the ability to pass on a foreign gene.
Aquaculture Microbiology and Biotechnology, Vol. 1 by Didier Montet, R.C. Ray