By Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)
Confocal microscopy with laser scanning know-how yields in-vivo pictures of ocular and ocular adnexal surfaces which are so amazing that they rival histology when it comes to quality.This special atlas and textbook demonstrates common in-vivo anatomy of the cornea, limbus and conjunctiva, quantifies a variety of mobile constructions utilizing cell-density calculations and establishes correlations among novel optical sections of varied ailments of the ocular floor and medical findings. additionally, it helps the translation of novel high-magnification optical sections through evaluating corneal and conjunctival imprint cytology with in-vivo pictures and describes early inflammatory alterations in corneal grafts, in addition to corneal conjunctivalisation in limbal stem phone deficiency, corneal dystrophies or infections, flap interface and margin features after laser in-situ keratomileusis (LASIK). furthermore, it instructs the reader approximately diagnostic and healing follow-up options and offers a quick advent to purposes in different fields similar to dentistry and ear, nostril and throat surgery.
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Additional info for Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology
15 Corneal erosion (fluorescence mode, contact examination). Fluorescein-stained superficial cells (up to 50 µm in size). In the left part of the picture (arrow), the borders of the smaller intermediate cells are visible Fig. 16 Corneal edema. Confocal images of central cornea. 2 Epithelium Fig. 17 Corneal infiltrate due to contact lens wear. Confocal images of transition area (from normal cornea to ulcer). a Edematous changes in epithelium and absence of cells in ulcer zone. b Massive leukocyte infiltration in transition area at the level of wing cells Fig.
Columnar basal cells have a flat basal surface adjacent to Bowman’s membrane, a frontal height of approximately 20 mm, and a frontal diameter of 8–10 mm. Like endothelial cells, they can be quantified accurately because of their defined location in relation to the basement membrane (Fig. 1). Bowman’s membrane – which is clearly distinct histologically from the epithelial basement membrane – is 10–16 mm thick and remains amorphous on light microscopy. Its location on in vivo confocal microscopy is well defined by the subepithelial plexus (SEP).
2 Epithelium a Fig. 31 Corneal epitheliopathy. a Slit-lamp photograph of a patient with severe ocular rosacea and conjunctival cell migration within the corneal epithelium. b Confocal in vivo microscopy image from the b same patient. Conjunctival cells (hyperreflective cells) migrating into an abnormal corneal epithelium (hyporeflective cells) a b Fig. 32 Corneal conjunctivalization in a 55-yearold woman as a complication of severe ocular rosacea. Partial keratinization and superior conjunctivalization (area 1) and severe corneal metaplasia (area 2).
Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology by Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)